Free Download FlowJo 10.10.2 (x64) | 336 Mb
FlowJo is a flow cytometry data analysis platform designed for processing and interpreting data generated by modern flow cytometers. It supports Flow Cytometry Standard (FCS) files and provides tools for organizing samples, creating gates, calculating statistics, generating graphical reports, and performing specialized analyses across complex experiments.
FlowJo organizes samples within workspaces using hierarchical views of populations, gates, statistics, tables, and layouts. Samples can be grouped by experimental attributes such as antibody panels, tissue types, or patients, allowing analyses to be applied consistently across multiple samples.
The software includes specialized platforms for cell cycle, kinetics, proliferation, compensation, population comparison, and other flow cytometry workflows. Batch operations, templates, backgating, derived parameters, and statistical tables help standardize repetitive analysis across groups of samples and experiments.
Software Overview
FlowJo is designed for analyzing complete flow cytometry experiments rather than isolated data files. Users can load FCS samples into workspaces, organize them into groups, create gating hierarchies, apply analyses across related samples, and generate graphical and statistical outputs.
Workspaces preserve gates, statistics, tables, and graphical layouts, allowing an experiment to be saved and reused as a template. The software also supports FCS file scanning, remote file locations, specialized analysis platforms, and data visualization through dynamic graphs and movies.
Key Features
- FCS Data Support: Import and analyze flow cytometry data stored in Flow Cytometry Standard (FCS) format.
- Workspaces: Organize samples, gates, statistics, tables, and graphical layouts within hierarchical workspaces.
- Sample Groups: Group samples by attributes such as antibody panels, tissue type, patient, or other experimental criteria.
- Gating Hierarchy: Create hierarchical populations and apply gates to parent and child subsets.
- Batch Operations: Apply repetitive analyses, layouts, and table generation across groups of samples.
- Templates: Save workspaces containing gates, statistics, tables, and layouts for reuse with subsequent experiments.
- Backgating: Display the source of a selected subpopulation throughout the gating hierarchy.
- Cell Cycle Analysis: Analyze G1, S, and G2 cell populations using multiple fitting models.
- Compensation: Create compensation matrices from singly stained controls and apply compensated parameters to multi-stained samples.
- Derived Parameters: Create scaling, time, ratio, and log-to-linear conversion parameters for analysis.
- Kinetics Analysis: Analyze time-dependent data including response time, response slope, and responding-cell fractions.
- Proliferation Analysis: Model proliferation data from cell tracking dyes such as CFSE and identify individual generations.
- Population Comparison: Assess statistical differences between populations across files.
- Plate Editor: Annotate, group, and organize plate-based flow cytometry data, including CSV-based plate annotation and titration setup.
- Statistics and Formulas: Calculate standard statistics across populations and groups and create custom formulas for repeated analysis.
- Data Tables: Build statistical tables across designated sample groups and export results to spreadsheets or databases.
- Graphical Reports: Create graphical layouts and detailed reports from selected analyses.
- Movies: Generate dynamic graphs for kinetic analyses or relationships between multiple parameters.
- FCS Scan: Search local or remote file systems for FCS files based on criteria such as stain protocol, operator ID, run date, or instrument.
- Data Hosting: Integrate remote data hosting services and support cloud deployment for high-throughput environments.
- Operating System: Windows and macOS
- Architecture: 64-bit
Code:
https://www.flowjo.com/- Software Name: FlowJo
- Version: 10.10.2
- Architecture: x64
- Languages: English
- License type: Full Version
- File Size: 336 Mb
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